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[1] Jackson WM, Nesti LJ, Tuan RS. Mesenchymal stem cell therapy for attenuation of scar formation during wound healing. Stem Cell Research & Therapy. 2012; 3: 20.

[2] National Institutes of Heatlh, US Department of Health and Human Services, Stem Cell Information, Basics [cited 2022 March 19]

[3] Boregowda S, Phinney D. Reconciling the stem cell and paracrine paradigms of mesenchymal stem cell function. In The Biology and Therapeutic Application of Mesenchymal Cells. Hoboken, New Jersey: John Wiley & Sons; published online 2016 Nov 26 [cited 2022 March 19]

[4] Izadpanah R, Trygg C, Patel B, Kriedt C, Dufour J, Gimble JM, Bunnell BA. Biologic properties of mesenchymal stem cells derived from bone marrow and adipose tissue. Journal of Cellular Biochemistry. 2006; 99(5): 1285–1297; Kern S, Eichler H, Stoeve J, Kluter H, Bieback K. Comparative analysis of mesenchymal stem cells from bone marrow, umbilical cord blood, or adipose tissue. Stem Cells. 2006; 24(5): 1294–1301; Jang Y, Koh YG, Choi YJ, Kim SH, Yoon DS, Lee M, Lee JW. Characterization of adipose tissue-derived stromal vascular fraction for clinical application to cartilage regeneration. Vitro Cellular & Developmental Biology-Animal. 2015 Feb 1; 51(2): 142–150; Zuk PA, Zhu M, Ashijan P, De Ugarte DA, Huang JI, Mizuno H, Alfonso ZC, Fraser JK, Benhaim P, Hedrick MH. Human adipose tissue is a source of multipotent stem cells. Molecular Biology of the Cell. 2002; 13(12): 4279–4295.

[5] Fraser JK, Wulur I, Alfonso Z, Hedrick MH. Fat tissue: An underappreciated source of stem cells for biotechnology. Trends in Biotechnology. 2006; 24(4): 150–154; Prockop DJ, Phinney DG, Bunnell BA. Mesenchymal Stem Cells: Methods and Protocols. Totowa, New Jersey: Humana Press, 2008.

[6] Beane OS, Fonseca VC, Cooper LL, Koren G, Darling EM. Impact of aging on the regenerative properties of bone marrow-, muscle-, and adipose-derived mesenchymal stem/stromal cells. PLOS ONE. 2014; 26(12) [cited 2022 March 19]

[7] Kim WS, Park BS, Sung JH, Yang JM, Park SB, Kwak SJ, Park SJ. Wound healing effect of adipose-derived stem cells: A critical role of secretory factors on human dermal fibroblasts.” Journal of Dermatological Science. 2007; 48(1): 15–24; Ebrahimian TG, Pouzoulet F, Squiban C, Buard V, Andre M, Cousin B, Gourmelon P, Benderitter M, Casteilla L, Tamarat R. Cell therapy based on adipose tissue-derived stromal cells promotes physiological and pathological wound healing. Arteriosclerosis, Thrombosis, and Vascular Biology. 2009; 29(4): 503–510.

[8] Little D, Guilak F, Ruch D. Ligament-derived matrix stimulates a ligamentous phenotype in human adipose-derived stem cells. Tissue Engineering Part A. 2010; 16(7): 2307–2319; Figueroa D, Espinosa M, Calvo R, Scheu M, Vaisman A, Gallegos M, Conget P. Anterior cruciate ligament regeneration using mesenchymal stem cells and collagen type I scaffold in a rabbit model. Knee Surgery, Sports Traumatology, Arthroscopy. 2014; 22(5): 1196–1202.

[9] Chen X, Zou X, Yin G, Ouyang H. Tendon tissue engineering with mesenchymal stem cells and biografts: An option for large tendon defects? Frontiers in Bioscience (Scholar Edition). 2009 Jun; 1: 23–32; Uysal AC, Mizuno H. Tendon regeneration and repair with adipose derived stem cells. Current Stem Cell Research & Therapy. 2010; 5(2): 161–167; Uysal, AC, Mizuno H. Differentiation of adipose-derived stem cells for tendon repair. Methods in Molecular Biology. 2011; 702: 443–451; Uysal AC, Mizuno H. Tendon regeneration and repair with adipose derived stem cells. Current Stem Cell Research & Therapy. 2010; 5(2): 161–167.

[10] Jung M, Kaszap B, Redöhl A, Steck E, Breusch S, Richter W, Gotterbarn T. Enhanced early tissue regeneration after matrix-assisted autologous mesenchymal stem cell transplantation in full thickness chondral defects in a minipig model. Cell Transplantation. 2009; 18(8): 923–932; Lee KB, Hui JH, Song IC, Ardany L, Lee EH. Injectable mesenchymal stem cell therapy for large cartilage defects: A porcine model. Stem Cells. 2007; 25(11): 2964–2971; Dragoo JL, Samimi B, Zhu M, Hame SL, Thomas BJ, Lieberman JR. Tissue-engineered cartilage and bone using stem cells from human infrapatellar fat pads. Journal of Bone & Joint Surgery (British volume). 2003; 85(5): 740–747; Gobbi A, Karnatzikos G, Sankineani SR. One-step surgery with multipotent stem cells for the treatment of large full-thickness chondral defects of the knee. American Journal of Sports Medicine. 2014; 42(3): 648–657.

[11] Hoogendoom RJW, Lu ZF, Kroeze RJ, Bank RA, Wuisman PI, Helder MN. Adipose stem cells for intervertebral disc regeneration: Current status and concepts for the future. Journal of Cellular and Molecular Medicine. 2008; 12(6A): 2205–2216; Richardson SM, Kalamegam G, Pushparaj PN, Matta C, Memic A, Khademhosseini A, Mobasheri R, Poletti FL, Hoyland JA, Mobasheri A. Mesenchymal stem cells in regenerative medicine: Focus on articular cartilage and intervertebral disc regeneration. Methods. 2016; 99(April): 69–80.

[12] Bacou F, El Andalousi RB, Daussin PA, Micallef JP, Levin JM, Chammas M, Casteilla L, Reyne Y, Nougues J. Transplantation of adipose tissue-derived stromal cells increases mass and functional capacity of damaged skeletal muscle. Cell Transplantation. 2004; 13(2): 103–111; Rodriguez LV, Alfonso Z, Zhang R, Leung J, Wu B, Ignarro LJ. Clonogenic multipotent stem cells in human adipose tissue differentiate into functional smooth muscle cells. Proceedings of the National Academy of Sciences of the United States of America. 2006; 103(32): 12167–12172; Goudenege S, Pisani DF, Wdziekonski B, Di Santo JP, Bagnis C, Dani C, Dechesne CA. Enhancement of myogenic and muscle repair capacities of human adipose-derived stem cells with forced expression of MyoD. Molecular Therapy. 2009; 17(6): 1064–1072.

[13] Murphy JM, Dixon K, Beck S, Fabian D, Feldman A, Barry F. Reduced chondrogenic and adipogenic activity of mesenchymal stem cells from patients with advanced osteoarthritis. Arthritis & Rheumatism. 2002; 46(3): 704–713; Luyten FP. Mesenchymal stem cells in osteoarthritis. Current Opinion in Rheumatology. 2004; 16(5): 599–603; Haynesworth SE, Kadiyala S, Liang LN, Thomas T, Bruder SP. Chemotactic and Mitogenic Stimulation of Human Mesenchymal Stem Cells by Platelet Rich Plasma Suggests a Mechanism for Enhancement of Bone Repair. Presented at the 48th Annual Meeting of the Orthopaedic Research Society, Dallas, Texas; February 10–13, 2002 [cited 2022 March 19]

[14] Wakitani S, Goto T, Pineda SJ, Young RG, Mansour JM, Caplan AI, Goldberg VM. Mesenchymal cell-based repair of large, full-thickness defects of articular cartilage. Journal of Bone & Joint Surgery (American volume). 1994; 76(4): 579–592; Wakitani S, Imoto K, Yamamoto T, Saito M, Murata N, Yoneda M. Human autologous culture expanded bone marrow mesenchymal cell transplantation for repair of cartilage defects in osteoarthritic knees. Osteoarthritis and Cartilage. 2002; 10(3): 199–206; Bui KHT, Pham PV, Duong TD, Nguyen NT, Nguyen TD, Le VT, Mai VT, Phan NLC, Le DM, Ngoc NK. Symptomatic knee osteoarthritis treatment using autologous adipose derived stem cells and platelet-rich plasma: A clinical study. Biomedical Research and Therapy. 2014; 1(1): 2–8.

[15] Filardo G, Perdisa F, Roffi A, Marcacci M, Kon E. Stem cells in articular cartilage regeneration. Journal of Orthopaedic Surgery and Research. 2016; 11(1): 1; Koh YG, Choi YJ, Kwon SK, Kim YS, Yeo JE. Clinical results and second-look arthroscopic findings after treatment with adipose-derived stem cells for knee osteoarthritis. Knee Surgery, Sports Traumatology, Arthroscopy. 2015; 23(5): 1308–1316.

[16] International Federation for Adipose Therapeutics and Science [cited 2016 January 24]

[17] Crisan M, Corselli M, Chen WC, Péault B. Perivascular cells for regenerative medicine. Journal of Cellular and Molecular Medicine. 2012; 16(12): 2851–2860.

[18] Malan T. Adipose-derived regenerative cells. In Stem Cells in Aesthetic Procedures (pp. 299–302). Berlin, Germany: Springer, 2014.

[19] Van Pham P, Bui KH, Ngo DQ, Vu NB, Truong NH, Phan NL, Le DM, Duong TD, Nguyen TD, Le VT, Phan NK. Activated platelet-rich plasma improves adipose-derived stem cell transplantation efficiency in injured articular cartilage. Stem Cell Research & Therapy. 2013; 4: 91.

[20] Blanton MW, Hadad I, Johnstone BH, Mund JA, Rogers PI, Eppley BL, March KL. Adipose stromal cells and platelet-rich plasma therapies synergistically increase revascularization during wound healing. Plastic and Reconstructive Surgery. 2009; 123(2 Suppl): 56S–64S; 91; Fukaya Y, Kuroda M, Aoyagi Y, Asada S, Kubota Y, Okamoto Y, Nakayama T, Saito Y, Satoh K, Bujo H. Platelet-rich plasma inhibits the apoptosis of highly adipogenic homogeneous preadipocytes in an in vitro culture system. Experimental & Molecular Medicine. 2012; 44(5): 330–339; Uysal CA, Tobita M, Hyakusoku H, Mizuno H. Adipose-derived stem cells enhance primary tendon repair: Biomechanical and immunohistochemical evaluation. Journal of Plastic, Reconstructive & Aesthetic Surgery. 2012; 65(12): 1712–1719.

[21] Uysal CA, Tobita M, Hyakusoku H, Mizuno H. Adipose-derived stem cells enhance primary tendon repair: Biomechanical and immunohistochemical evaluation. Journal of Plastic, Reconstructive & Aesthetic Surgery. 2012; 65(12): 1712–1719.

[22] Wyles CC, Houdek MT, Behfar A, Sierra, RJ. Mesenchymal stem cell therapy for osteoarthritis: current perspectives. Stem Cells and Cloning: Advances and Applications. 2015; 8: 117–124.

[23] van Lent, PL, van den Berg, WB. Mesenchymal stem cell therapy in osteoarthritis: Advanced tissue repair or intervention with smouldering synovial activation? Arthritis Research & Therapy. 2013 Mar 20; 15(2): 112.

[24] Wolfstadt JI, Cole BJ, Ogilvie-Harris DJ, Viswanathan S, Chahal J. Current concepts: The role of mesenchymal stem cells in the management of knee osteoarthritis. Sports Health. 2015; 7(1): 38–44.

[25] Buckwalter, JA, Saltzman C, Brown T. The impact of osteoarthritis: Implications for research. Clinical Orthopaedics and Related Research. 2004; 427: S6–S15.

[26] Ruetze M, Richter W. Adipose-derived stromal cells for osteoarticular repair: Trophic function versus stem cell activity. Expert Reviews in Molecular Medicine. 2014 May [cited 2017 October 1]; Reich CM, Raabe O, Wenisch S, Bridger PS, Kramer M, Arnhold S. Isolation, culture and chondrogenic differentiation of canine adipose tissue- and bone marrow-derived mesenchymal stem cells: A comparative study. Veterinary Research Communications. 2012; 36(2): 139–148; Afizah H, Yang Z, Hui JH, Ouyang HW, Lee EH. A comparison between the chondrogenic potential of human bone marrow stem cells (BMSCs) and adipose-derived stem cells (ADSCs) taken from the same donors. Tissue Engineering. 2007 April; 13(4): 659–666.

[27] Dragoo JL, Carlson G, McCormick F, Khan-Farooqi H, Zhu M, Zuk PA, Benhaim P. Healing full-thickness cartilage defects using adipose-derived stem cells. Tissue Engineering. 2007; 13(7): 1615–1621.

[28] Im GI, Shin YW, Lee KB. Do adipose tissue-derived mesenchymal stem cells have the same osteogenic and chondrogenic potential as bone marrow-derived cells? Osteoarthritis and Cartilage. 2005; 13: 845–53.

[29] Freitag J, Shah K, Wickham J, Boyd R, Tenen A. The effect of autologous adipose derived mesenchymal stem cell therapy in the treatment of a large osteochondral defect of the knee following unsuccessful surgical intervention of osteochondritis dissecans–a case study. BMC Musculoskeletal Disorders. 2017 Jul 14; 18(1): 298.

[30] Melief SM, Zwaginga JJ, Fibbe WE, Roelofs H. Adipose tissue-derived multipotent stromal cells have a higher immunomodulatory capacity than their bone marrow-derived counterparts. Stem Cells Translational Medicine. 2013; 2(6): 455–463.

[31] Oshita T, Tobita M, Tajima S, Mizuno H. Adipose-derived stem cells improve collagenase-induced tendinopathy in a rat model. American Journal of Sports Medicine. 2016 Aug; 44(8): 1983–1989: Choi YS, Vincent LG, Lee AR, Dobke MK, Engler AJ. Mechanical derivation of functional myotubes from adipose-derived stem cells. Biomaterials. 2012 Mar; 33(8): 2482–2491; De Francesco F, Ricci G, D’Andrea F, Nicoletti GF, Ferraro GA. Human adipose stem cells: from bench to bedside. Tissue Engineering Part B: Reviews. 2015; 21(6): 572–584; Obaid, H, Connell D. Cell therapy in tendon disorders: What is the current evidence? American Journal of Sports Medicine. 2010; 38(10): 2123–2132.

[32] Adriani E, Moio M, Di Paola B, Salustri W, Alfieri A, Parisi P, Ruggiero M, Borab Z, Carlesimo B. Percutaneous Fat Transfer to Treat Knee Osteoarthritis Symptoms: Preliminary Results. Joints. 2017 Jun; 5(2): 89–92.

[33] Tangchitphisut P, Srikaew N, Numhom S, Tangprasittipap A, Woratanarat P, Wongsak S, Kijkunasathian C, Hongeng S, Murray IR, Tawonsawatruk T. Infrapatellar Fat Pad: An alternative source of adipose-derived mesenchymal stem cells. Arthritis. 2016 Apr 26 [cited 2022 March 19] English A, Jones EA, Corscadden D, Henshaw K, Chapman T, Emery P, McGonagle D. A comparative assessment of cartilage and joint fat pad as a potential source of cells for autologous therapy development in knee osteoarthritis. Rheumatology. 2007 Sep 26; 46(11): 1676–1683.

[34] Hindle P, Khan N, Biant L, Péault B. The Infrapatellar fat pad as a source of perivascular stem cells with increased chondrogenic potential for regenerative medicine. Stem Cells Translational Medicine. 2017; 6(1): 77–87.

[35] Teichtahl AJ, Wulidasari E, Brady SRE, Wang Y, Wluka AE, Ding C, Giles GG, Cicuttini FM. A large infrapatellar fat pad protects against knee pain and lateral tibial cartilage volume loss. Arthritis Research & Therapy. 2015; 17(1): 318.

[36] Adriani E, Moio M, Di Paola B, Salustri W, Alfieri A, Parisi P, Ruggiero M, Borab Z, Carlesimo B. Percutaneous Fat Transfer to Treat Knee Osteoarthritis Symptoms: Preliminary Results. Joints. 2017 Jun; 5(2): 89–92.

[37] Ruhdorfer A, Haniel F, Petersohn T, Dörrenberg J, Wirth W, Dannhauer T, Hunter DJ, Eckstein F. Between-group differences in infra-patellar fat pad size and signal in symptomatic and radiographic progression of knee osteoarthritis vs non-progressive controls and healthy knees–data from the FNIH Biomarkers Consortium Study and the Osteoarthritis Initiative. Osteoarthritis and Cartilage. 2017; 25(7): 1114–1121.

[38] Cowan, S.M., H.F. Hart, S.J. Warden, and K.M. Crossley. Infrapatellar fat pad volume is greater in individuals with patellofemoral joint osteoarthritis and associated with pain. Rheumatology International. August 2015; 35(8): 1439–1442; Clockaerts S, Bastiaansen-Jenniskens YM, Runhaar J, Van Osch GJ, Van Offel JF, Verhaar JA, De Clerck LS, Somville J. The infrapatellar fat pad should be considered as an active osteoarthritic joint tissue: A narrative review. Osteoarthritis and Cartilage. 2010 Jul 31; 18(7): 876–882.

[39] Antony BSE, Jin X, Wang X, Han W, Cicuttini F, Ding C, Jones G. OP0144 Infra patellar fat pad signal intensity alterations and effusion-synovitis predict knee pain, cartilage volume loss and total knee replacement over 10.7 years in older adults. Annals of the Rheumatic Diseases. 2016; 75(2): 110–111.

[40] Gwyn R, Kotwal RS, Holt MD, Davies AP. Complete excision of the infrapatellar fat pad is associated with patellar tendon shortening after primary total knee arthroplasty. European Journal of Orthopaedic Surgery & Traumatology. 2016 Jul 1; 26(5): 545–549.

[41] Hernigou P, Homma Y, Flouzat-Lachaniette CH, Poignard A, Chevallier N, Rouard H. Cancer risk is not increased in patients treated for orthopaedic diseases with autologous bone marrow cell concentrate. Journal of Bone & Joint Surgery (American Volume). 2013 Dec; 95(24): 2215–2221.

[42] Pak J, Chang JJ, Lee JH, Lee SH. Safety reporting on implantation of autologous adipose tissue-derived stem cells with platelet-rich plasma into human articular joints. BMC Musculoskeletal Disorders. 2013; 14: 337.

[43] Hatzistergos KE, Blum A, Ince T, Grichnik JM, Hare JM. What is the oncologic risk of stem cell treatment for heart disease? Circulation Research. 2011; 108(11): 1300–1303; Heldman AW, DiFede DL, Fishman JE, Zambrano JP, Trachtenberg BH, Karantalis V, Mushtaq M, Williams AR, Suncion VY, McNiece IK, Ghersin E. Transendocardial mesenchymal stem cells and mononuclear bone marrow cells for ischemic cardiomyopathy: The TAC-HFT randomized trial. JAMA. 2014; 311(1): 62–73.

[44] Filardo G, Perdisa F, Roffi A, Marcacci M, Kon E. Stem cells in articular cartilage regeneration. Journal of Orthopaedic Surgery and Research. 2016 Apr 12; 11:42, 15 pages.

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